molecular detection of class-d oxa carbapenemase genes in biofilm and non-biofilm forming clinical isolates of acinetobacter baumannii

conclusions coexistence of the blaoxa-51, blaoxa-23 and blaoxa-24/40 like genes, along with formation of strong biofilm, in mdr-ab strains particularly with indiscriminate use of imipenem, complicated treatment of the patients infected with these bacteria in the hospitals understudy. background emergence and spread of carbapenemase (blaoxa) genes in multidrug resistant acinetobacter baumannii (mdr-ab) forming biofilm complicated treatment of the patients infected with this microorganism particularly in intensive care units (icus). objectives the current study aimed to determine the prevalence of molecular class-d oxa carbapenemase in biofilm and non-biofilm forming strains of mdr-ab. materials and methods a total of 65 strains of mdr-ab were isolated from the patients hospitalized in the icu of two hospitals in kerman, iran. the isolates were identified by conventional microbiological tests as well as api 20ne assay. antibiotic susceptibility was carried out by disk diffusion method; minimum inhibitory concentration (mic) of carbapenems was measured by e-test. the presence of blaoxa genes among the isolates were studied by duplex-polymerase chain reaction and application of appropriate primers. biofilm formation was detected by microtiter plate method. results the isolates were highly resistant to ciprofloxacin, levofloxacin, piperacillin, nalidixic acid and third generation cephalosporins such as tigecycline (7%; n = 5) and colistin (13%; n = 8). among the isolates, 77% (n = 50) exhibited high mic (265μg/ml) for imipenem. both the blaoxa-51 and blaoxa-23 like genes coexisted in all the isolates; while, blaoxa-24/40 like gene was only detected in 29 imipenem-resistant strains (p ≤ 0.05). the blaoxa-58 like gene was not detected among the isolated strains. quantification of biofilm introduced 23 isolates (including blaoxa-24/40 strains) with efficient attachment to microtiter plate; while, those isolates without blaoxa-24/40, or imipenem-sensitive strains formed weak or no biofilm.